OK, here’s a work in progress, I’ll edit this until I think I’ve got it right at which point I’ll delete this paragraph and add a second post to notify anyone watching this thread.
Bill of Materials
- 1x Pioreactor + Raspberry Pi: Pioreactor 20ml | Pioreactor [1]
- 1x Dovetail platform for Raspberry Pi [2]
CO2 sparging
- 1x Sodastream cylinder: Amazon.co.uk (classic blue NOT quick connect pink)
- 1x Dovetail Sodastream cylinder holder - @SandrineH 's Design on Printables
- 1x W21.8-14 to TR21-4 adaptor: Amazon.co.uk [3]
- 1x CO2 Regulator and Solenoid: Amazon.co.uk [4]
- ~ 20cm 4mm PU tubing: Amazon.co.uk
- 2x Check valves: Amazon.co.uk
- 1x 3/16" barb male Luer lock: Amazon.co.uk
- 1/16" barb Luer Locks - Male: Amazon.co.uk & Female: Amazon.co.uk
- ~ 20cm 1/16" ID Silicone tubing: Amazon.co.uk
- 1x 2.1mm x 5.5mm Male Barrel Plug: Amazon.co.uk - to connect Raspberry Pi to solenoid with:
- 4x Female Dupont Cables: Amazon.co.uk (also needed for electrolysis)
Electrolysis
- 4 port + 2 M4 threads vial cap - @RobJ 's Design on Printables
- 2x Stainless Steel 70mm M4 bolts: Amazon.co.uk
- 2x Stainless Steel M4 Locking Nuts (I got mine in a set: Amazon.co.uk)
- 2x Ring Crimp Terminals: Amazon.co.uk [5]
- 1 strip cut from a Platinized Titanium Anode Please let us know below if you’re considering purchasing an electrode, we’re currently doing lots of work on this
Reagents
Future requirements (not used in the above video)
To allow continuous operation (chemostat/turbidostat) once the culture is established:
- 2x Peristaltic pumps: 12V peristaltic pumps | Pioreactor
- 2x Dovetail peristaltic pump holder
- 2x Centrifuge tubes: Amazon.co.uk
- 2x Dovetail centrifuge tube holders
To monitor colour (absolutely optional, possibly not even useful, watch this space)
- 1x Adafruit Spectrometer: Amazon.co.uk
- 1x Stemma QT cable: Amazon.co.uk
Other extras
- Spare 20ml glass vials: Amazon.co.uk
Tools
- 1x 1-5ml Pipettor & tips (I also got a 10-100 ul pipettor and tips but didn’t use in this experiment)
- 1x Crimping Pliers: Amazon.co.uk - both for crimping and bolt cutting
- 1x 150mm Pliers Wrench: Amazon.co.uk
- Swiss Army Knife Pliers
- 1x Nitrile Gloves: Amazon.co.uk
- 1x PH0 Screwdriver: Amazon.co.uk
- 1x Spatula: Amazon.co.uk
- 1x Weighing Boat: Amazon.co.uk
- Jewellers Scales
- 1x 1L volumetric flask: Labpals
- 1x 1L Reagent Bottle: Labpals
- 1x 1L Amber Reagent Bottle: Labpals
- 1x 1L Wash Bottle: Labpals
I used a Raspberry Pi 4: Raspberry Pi 4 Model B - 8GB RAM but only because my studio setup requires ethernet rather than WiFi ↩︎
Actually mine was modified by @CamDavidsonPilon to fit the Ethernet cable (Cam, is that mod available online? Answer: yes here) ↩︎
Could be improved, I got this as it is also a 90° bend, but mine is uncomfortably stiff to adjust ↩︎
Would we be better off with a pinch valve? Can we produce an open source pinch valve based on a widely available solenoid? If so, could a 5v solenoid suffice? That would mean we don’t need a second power adapter ↩︎
I don’t think I’d recommend the crimp terminal set I bought ↩︎