HOB Culture Medium Ingredients

OK, unless anyone disagrees, I’m about to make up the following:

  1. 0.56 g/L NH4HCO3 1 - Entirely Ingredients
  2. 1.05 g/L NaHCO3 - Dr Oetker
  3. 2.3 g/L KH2PO4 - APC Pure
  4. 2.9 g/L Na2HPO4 - APC Pure
  5. 0.5 g/L MgSO4⋅7 H2O - Health Leads Epsom Salts
  6. 0.01 g/L CaCl2 - Intralabs
  7. 1 pill/L Vegan Multivitamins & Minerals with High B12, D3 & K2 - Vegan Vitality 2
  8. Make up to 1L with raw water 3

The next big question is then whether I should start the culture with 100% culture media rather than adding it gradually to a sample of pure raw water that we electrolyse. I'm thinking the former otherwise the ionic strength may be too low for successful electrolysis.


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1 to give the same mass of NH4 as 0.47 g/L (NH4)2SO4 - please check my maths
2 see https://forum.amybo.org/d/25-trace-element-solution/3 for further discussion of trace minerals
3 rather than risk washing out the bugs should the chemostat flow be too high, I'm thinking of making up the initial culture media with the raw water that we're enriching.