# Pioflo.org - enabling affordable bioreactors at scale **URL:** \ **Category:** Pioreactor\ **Created:** [11 November 2024 17:10 UTC](https://forum.amybo.org/t/pioflo-org-enabling-affordable-bioreactors-at-scale/91 "2024-11-11T17:10:57Z")\ **Posts on this page:** 4\ **Page:** 1
**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\ **Post date:** [11 November 2024 17:10 UTC](https://forum.amybo.org/t/pioflo-org-enabling-affordable-bioreactors-at-scale/91/1 "2024-11-11T17:10:57Z")
We produced the concept of a flow through cell version of a Pioreactor which could therefore control any size of Pioreactor for a [UK-Kenya Alternative Proteins Global Innovation Network](https://iuk-business-connect.org.uk/programme/uk-kenya-alternative-proteins-global-innovation-network/) grant application and managed a rather nice TRL4 Kombucha demonstrator. We set it as a [Harvey Mudd Clinic](https://www.hmc.edu/clinic/) stretch goal, but on reading @gerrit 's [From Pioreactor to FlavorReactor](https://forum.amybo.org/t/from-pioreactor-to-flavorreactor/88/1) post, I thought I’d better start writing up our work to date at [pioflo.org](http://pioflo.org) - does it make sense? And what should we do next? ---
**Author:** ![gerrit](https://forum.amybo.org/user_avatar/forum.amybo.org/gerrit/32/3_2.png) [@gerrit](https://forum.amybo.org/u/gerrit)\ **Post date:** [12 November 2024 11:23 UTC](https://forum.amybo.org/t/pioflo-org-enabling-affordable-bioreactors-at-scale/91/2 "2024-11-12T11:23:29Z")
Nice write-up of the kombucha experiment over on the wiki, @Martin ! So instead of using a second peristaltic pump to move the liquid back, you let it overflow through the tubes? Interesting. Would you mind sharing your Pioreactor setup/configuration on the wiki too, e.g. were you running the pump constantly, or at certain intervals? ---
**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\ **Post date:** [12 November 2024 11:47 UTC](https://forum.amybo.org/t/pioflo-org-enabling-affordable-bioreactors-at-scale/91/3 "2024-11-12T11:47:09Z")
Thanks @gerrit, yes all good points: I was actually using a pump to move the liquid back, then letting the suction from this draw in the kombucha from the beaker. I’ll clarify that in the writeup. And yes, I intend to add to it both with the Pioreactor setup/config (the pump was running at intervals, but I couldn’t remember what they were & need to get back to the lab to check) and with the later data which showed some more interesting results. I wonder on the latter whether I should do a video first to avoid spoilers… The big question is how do we best scale this next. I think the biggest difference will be tubing diameter between the CSTR and the Pioreactor. Step 1 would be to use as large bore tubing as could be accommodated on a 20ml vial cap. Step 2 would be going full bore, pumping through the Pioflo from bottom to top. Step 2 would clearly require significant physical modifications to the Pioreactor. In order to avoid buying redundant kit, I’d next like to get a pump that the Pioreactor can control for Step 1 that could also accommodate full bore tubing for Step 2. ---
**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\ **Post date:** [25 November 2024 12:42 UTC](https://forum.amybo.org/t/pioflo-org-enabling-affordable-bioreactors-at-scale/91/4 "2024-11-25T12:42:29Z")
We’re hosting a meeting for everyone working on Larger Pioreactors at 17:00 UTC on 28 November. DM me if you’d like an invite, or if you can’t make it but would like to stay in the loop.