# How to calibrate optical density

**URL:** <https://forum.amybo.org/t/how-to-calibrate-optical-density/156>\
**Category:** electroPioreactor\
**Created:** [25 January 2026 14:54 UTC](https://forum.amybo.org/t/how-to-calibrate-optical-density/156 "2026-01-25T14:54:44Z")\
**Posts on this page:** 2\
**Page:** 1

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**Author:** ![Bingqiao](https://forum.amybo.org/letter_avatar_proxy/v4/letter/b/bc79bd/32.png) [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\
**Post date:** [25 January 2026 14:54 UTC](https://forum.amybo.org/t/how-to-calibrate-optical-density/156/1 "2026-01-25T14:54:44Z")

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Hi! Could anyone tell me how to calibrate optical density please? I did not find that in pioreactor docs.

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**Author:** ![Bingqiao](https://forum.amybo.org/letter_avatar_proxy/v4/letter/b/bc79bd/32.png) [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\
**Post date:** [25 January 2026 14:58 UTC](https://forum.amybo.org/t/how-to-calibrate-optical-density/156/2 "2026-01-25T14:58:21Z")

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Sorry, my bad. I found that. It is here, [Standard curves for OD600 readings | Pioreactor Docs](https://docs.pioreactor.com/user-guide/calibrate-od600)