# HOB Culture Medium Ingredients **URL:** \ **Category:** Bugs\ **Created:** [22 December 2023 19:11 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53 "2023-12-22T19:11:35Z")\ **Posts on this page:** 1\ **Showing post:** 7
**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\ **Post date:** [2 January 2024 22:24 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53/7 "2024-01-02T22:24:45Z")
OK, unless anyone disagrees, I’m about to make up the following: 1. 0.56 g/L NH4HCO3 1 - [Entirely Ingredients](https://amzn.eu/d/hBfaVPj) 2. 1.05 g/L NaHCO3 - [Dr Oetker](https://www.tesco.com/groceries/en-GB/products/282996938) 3. 2.3 g/L KH2PO4 - [APC Pure](https://apcpure.com/product/potassium-dihydrogen-phosphate-99-102-00-anhydrous-acs-fg/) 4. 2.9 g/L Na2HPO4 - [APC Pure](https://apcpure.com/product/di-sodium-hydrogen-phosphate-anhydrous-98-5-acs-food-grade/?attribute_pack-size=100gm) 5. 0.5 g/L MgSO4⋅7 H2O - [Health Leads Epsom Salts](https://amzn.eu/d/49Etx5x) 6. 0.01 g/L CaCl2 - [Intralabs](https://amzn.eu/d/21TBBSU) 7. 1 pill/L Vegan Multivitamins & Minerals with High B12, D3 & K2 - [Vegan Vitality](https://vegan-vitality.co.uk/collections/frontpage/products/vegan-multivitamin?variant=20464099688512) 2 8. Make up to 1L with raw water 3 The next big question is then whether I should start the culture with 100% culture media rather than adding it gradually to a sample of pure raw water that we electrolyse. I'm thinking the former otherwise the ionic strength may be too low for successful electrolysis. * * * --- 1 to give the same mass of NH4 as 0.47 g/L (NH4)2SO4 - please check my maths 2 see https://forum.amybo.org/d/25-trace-element-solution/3 for further discussion of trace minerals 3 rather than risk washing out the bugs should the chemostat flow be too high, I'm thinking of making up the initial culture media with the raw water that we're enriching. --- _[View the full topic](https://forum.amybo.org/t/hob-culture-medium-ingredients/53)._