# HOB Culture Medium Ingredients **URL:** \ **Category:** Bugs\ **Created:** [22 December 2023 19:11 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53 "2023-12-22T19:11:35Z")\ **Posts on this page:** 1\ **Showing post:** 12
**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\ **Post date:** [2 April 2025 03:47 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53/12 "2025-04-02T03:47:45Z")
A long overdue update, but the experiment using a multivitamin & mineral pill was (not surprisingly) unsuccessful. I’d currently recommend anyone trying an HOB enrichments starts out by trying to replicate successful ones in the literature like @NarcisP’s [Electricity-driven microbial protein production: Effect of current density on biomass growth and nitrogen assimilation](https://pdf.sciencedirectassets.com/282892/1-s2.0-S2213343723X00062/1-s2.0-S2213343723022893/main.pdf?X-Amz-Security-Token=IQoJb3JpZ2luX2VjEGMaCXVzLWVhc3QtMSJGMEQCIHMxRQ6sb6T4YDBMs9bPuxXP%2FtJtVTpENHPP6NVZyuFAAiB3t3YR0y7DI81buVf4GXL2U1NzH1uMaH0EfPpoBseq0Cq8BQjM%2F%2F%2F%2F%2F%2F%2F%2F%2F%2F8BEAUaDDA1OTAwMzU0Njg2NSIMCouJuKStFMf%2BdnlJKpAFY6Q0McGP7rMZNUl%2FkFJ3zvBk7rrYBxDIQIKkMpdudP6rsZqspMU4gccYN0p51CMd6cI9yk0am1PpYzZOn9UHqIXSRhpK%2BS%2FN91qfB1wVgk7OiBIbeitdCp3%2F6dvJHraVELxqYmX9x26qDFhYm9R3BBuemz7AaHK2U1uZPZxi3l%2FCmuhCiHz8HO0U1DXA3aK0JlHhvGtH%2FYJJFzJnilmSqXObMC1I4v8Ht%2FBxniwPiHE0D9GXNW1X4ZUJDHl6PxBmaTSTZ5IokG8GYbNt%2FWCOtm%2FgmLoJBnyk697jO7rrfs9AteTcY4ky9SldeRp1ZDeVjWMgfMhDFyru08iVJmWLnYkjwkUnF6FFqE49QWSMSxifT%2BnXqtaFLRpzZ71K1zPdKiwXRNOBvvivDyFev8IBzHBvJ9X%2FfIl1dWb4N5IrFQ9IG%2FxglkD9pHP7MThpjCd3x78tZjBgm6PWvxzS6PSQaicIQ6GxAnyiTTqg%2FVGJu7EjA5NzV%2FeSM9LwjiJojA9kFenfJvqrb2ORZ06SbMK%2FRG6k4t%2BPT%2BLXhPhjpMUt8DasSGymrIfIsPBGVLfAbkzOG9GNruq4M20FRRymDnqZmJF7KKd%2BNg%2Fp7RbdmDYap8gRA05kb6%2FDur1GtdfqFaERRrU2MT8nIcBDbNPxLlCZe12Nc1JYhNrBmK%2Fq3wTyC8xeaPEtlTlfZZrLd%2FP16SB04JA4tFNGIZlYwxT0GMfAa7%2FKaSFwRsDLrD%2B%2BRpHKwbtAEswvKLTU3VVPV9ZGSAv6qR5UATH711ACZ%2Fpg6lTQzkZv9s7RFi250E7g8GvyZb843lIqQOk61IozMSc%2FPZhDQmDilWW9wUJmgkH72i40xalCW2P9PJLDIdntJwMnCQow3cCyvwY6sgHXD0vS1H7pC2S3Tx1gLoa2CRi2kRCOw9PagAXwcvjko3tzAspvEwJ4w5Pf9HQorZrlB1Piw1VvMAd2CUIp2adARIRTmS%2FppUFB9SRmYW6UGkF01RsdrK74E7QoiZqncN8l9VQAZSaslULhHct6Pek15dfzU9pT2UW3XOMc5oc5aIyL8%2F5nuaq1CCDgjXw1rKtxniLh0FZW3l6900e3B0Srv9IqW7beyBTS2JZjTboQrYBN&X-Amz-Algorithm=AWS4-HMAC-SHA256&X-Amz-Date=20250402T034324Z&X-Amz-SignedHeaders=host&X-Amz-Expires=300&X-Amz-Credential=ASIAQ3PHCVTY6VOP7LWD%2F20250402%2Fus-east-1%2Fs3%2Faws4_request&X-Amz-Signature=e487cc3eef19baedc766888537f6f2b38074591c85b7106b61afc6d9be49c980&hash=fb10aa40eba35290ebe3b8c9ed214a057b6ccfa1c0a6f39751f6b4d6dbe8a4b4&host=68042c943591013ac2b2430a89b270f6af2c76d8dfd086a07176afe7c76c2c61&pii=S2213343723022893&tid=spdf-ae019ecd-427a-48b1-8082-ec191efea3e4&sid=329bd51f99c4304a5b4a3d361bf99dfc2c6bgxrqb&type=client&tsoh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&rh=d3d3LnNjaWVuY2VkaXJlY3QuY29t&ua=1d035b555e0054515555&rr=929d52610c18cc92&cc=gb). Papers we have found to date use [Rabaey](https://iwaponline.com/wst/article-abstract/52/1-2/515/13027/Continuous-microbial-fuel-cells-convert?redirectedFrom=fulltext), [Yu](https://www.mdpi.com/2311-5637/4/3/63) or [SL-6](https://bacmedia.dsmz.de/solutions/3822) for their micronutrients. They use various mesonutrients similar, but not identical, to the ones I tried above. See also @arlombardo’s [post](https://forum.amybo.org/t/keeping-up-with-hmc-clinic-project/126/34) which alerted me to this oversight. --- _[View the full topic](https://forum.amybo.org/t/hob-culture-medium-ingredients/53)._