# HOB Culture Medium Ingredients

**URL:** <https://forum.amybo.org/t/hob-culture-medium-ingredients/53>\
**Category:** Bugs\
**Created:** [22 December 2023 19:11 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53 "2023-12-22T19:11:35Z")\
**Posts on this page:** 1\
**Showing post:** 11

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**Author:** ![Martin](https://forum.amybo.org/user_avatar/forum.amybo.org/martin/32/19_2.png) [@Martin](https://forum.amybo.org/u/Martin)\
**Post date:** [5 April 2024 10:00 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53/11 "2024-04-05T10:00:12Z")

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Well spotted. I’d want to avoid Chlorides in these mesonutrients at least (see @NarcisP’ [reply here](https://forum.amybo.org/t/trace-element-solution/56/21)) although I’ve just checked that they used CaCl2 according to their 2023 paper\[1\] I have a feeling that avoiding chlorides altogether may increase yield (unless of course the bugs actually need chloride in which case we’re stuck with a balance of meeting that need and bleaching them.

[1] N. Pous, M. D. Balaguer, P. Chiluiza-Ramos, L. Rovira-Alsina, L. Bañeras, and S. Puig, ‘Electricity-driven microbial protein production: Effect of current density on biomass growth and nitrogen assimilation’, _Journal of Environmental Chemical Engineering_, vol. 11, no. 6, p. 111550, Dec. 2023, doi: [10.1016/j.jece.2023.111550](https://doi.org/10.1016/j.jece.2023.111550).

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