# Latest **URL:** https://forum.amybo.org/latest.md [Latest](https://forum.amybo.org/latest.md) · [Categories](https://forum.amybo.org/categories.md) · [Tags](https://forum.amybo.org/tags.md) --- ## [Welcome to AMYBO.org! 👋](https://forum.amybo.org/t/welcome-to-amybo-org/5)
**Author:** [@system](https://forum.amybo.org/u/system)\ **Replies:** 0\ **Last updated:** [21 February 2024 15:45 UTC](https://forum.amybo.org/t/welcome-to-amybo-org/5 "2024-02-21T15:45:59Z")
We are so glad you joined us. AMYBO.org A non-profit open source protein fermentation community Here are some things you can do to get started: :speaking\_head: Introduce yourself by adding your picture and informati… --- ## [AMYBO London Event - 13 Nov](https://forum.amybo.org/t/amybo-london-event-13-nov/167)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 0\ **Last updated:** [13 October 2026 10:11 UTC](https://forum.amybo.org/t/amybo-london-event-13-nov/167 "2026-10-13T10:11:49Z")
Hi All We’re delighted to announce our first AMYBO official get-together at Imperial College’s new White City Labs on 13 November. Join us for a tour of the labs, a first public reveal of the new Aseptic electroPior… --- ## [What license should work done by Amybo be placed under?](https://forum.amybo.org/t/what-license-should-work-done-by-amybo-be-placed-under/47)
**Author:** [@RobJ](https://forum.amybo.org/u/RobJ)\ **Replies:** 25\ **Last updated:** [9 September 2026 09:20 UTC](https://forum.amybo.org/t/what-license-should-work-done-by-amybo-be-placed-under/47 "2026-09-09T09:20:08Z")
I was wondering what licenses people think are best for the work done by Amybo? As far as hardware goes, I think the CERN-OHL-P or the MIT license may be good options. CC0 1.0 or CC BY 4.0 are also worth taking a look… --- ## [Current measurement experiment](https://forum.amybo.org/t/current-measurement-experiment/166)
**Author:** [@gerrit](https://forum.amybo.org/u/gerrit)\ **Replies:** 4\ **Last updated:** [3 June 2026 15:34 UTC](https://forum.amybo.org/t/current-measurement-experiment/166 "2026-06-03T15:34:20Z")
I wanted to see whether a given intensity setting on the Pioreactor’s LED channels produces the same current regardless of the solution used, so I tried different electrolyte solutions and measured the current and bus vo… --- ## [CO2 sparger drawback](https://forum.amybo.org/t/co2-sparger-drawback/52)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 15\ **Last updated:** [2 June 2026 18:53 UTC](https://forum.amybo.org/t/co2-sparger-drawback/52 "2026-06-02T18:53:12Z")
I could do with a quick sanity check, so I’m tagging you @Trusted as I have 2h to place an order that may fix things in time for tomorrow’s video. I set up an FZONE CO2 Regulator and Solenoid on an adaptor on a Sodastre… --- ## [phD and General Inquiry](https://forum.amybo.org/t/phd-and-general-inquiry/165)
**Author:** [@nunyabuya](https://forum.amybo.org/u/nunyabuya)\ **Replies:** 5\ **Last updated:** [29 May 2026 02:29 UTC](https://forum.amybo.org/t/phd-and-general-inquiry/165 "2026-05-29T02:29:07Z")
Hey, i submitted my application for the posted PhD program but wanted to follow here as well. I hoped to start a conversation about amybo as I just found some videos posted 2 years ago regarding the pioreactor. I think t… --- ## [PhD - developing the Aseptic electroPioreactor (and much more)](https://forum.amybo.org/t/phd-developing-the-aseptic-electropioreactor-and-much-more/164)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 0\ **Last updated:** [20 May 2026 16:12 UTC](https://forum.amybo.org/t/phd-developing-the-aseptic-electropioreactor-and-much-more/164 "2026-05-20T16:12:51Z")
Know someone interested in a PhD in Sustainable Protein? I’m delighted to be Industry Supervisor of a PhD with the wonderful Sonja Billerbeck at the Bezos Centre for Sustainable Protein - Imperial. It’s fully funded fo… --- ## [Splash of media with K-acetate on pioreactor](https://forum.amybo.org/t/splash-of-media-with-k-acetate-on-pioreactor/161)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 6\ **Last updated:** [25 April 2026 02:35 UTC](https://forum.amybo.org/t/splash-of-media-with-k-acetate-on-pioreactor/161 "2026-04-25T02:35:29Z")
I had a media splash ( electroPioreactor/Media at main · amy-bo/electroPioreactor · GitHub + Medan potassium acetate). Prior to realising that I must power off, I was getting green and yellow lights on the RPi but the b… --- ## [Trial experiment on heterotrophic electrolysis](https://forum.amybo.org/t/trial-experiment-on-heterotrophic-electrolysis/163)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 3\ **Last updated:** [2 April 2026 22:22 UTC](https://forum.amybo.org/t/trial-experiment-on-heterotrophic-electrolysis/163 "2026-04-02T22:22:38Z")
Methodology: Setting the pioreactor and experiment environment (no media exchange during experiment) Ed01: 4%;no chloride media; initial OD600 0.05 CH34 Ed02: 3.5%; no chloride media; initial OD600 0.05 CH34 Ed03: 3… --- ## [Media with acidified meso nutrient may not support the growth](https://forum.amybo.org/t/media-with-acidified-meso-nutrient-may-not-support-the-growth/160)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 2\ **Last updated:** [25 February 2026 14:29 UTC](https://forum.amybo.org/t/media-with-acidified-meso-nutrient-may-not-support-the-growth/160 "2026-02-25T14:29:20Z")
My recent work utilised meso-nutrient, iron, potassium acetate, and acidified trace elements, but these do not support growth in our pioreactor. It happened to all of my reactors, but it worked in my flask in the incubat… --- ## [Rebooting Journal Club?](https://forum.amybo.org/t/rebooting-journal-club/145)
**Author:** [@gerrit](https://forum.amybo.org/u/gerrit)\ **Replies:** 33\ **Last updated:** [21 February 2026 16:13 UTC](https://forum.amybo.org/t/rebooting-journal-club/145 "2026-02-21T16:13:45Z")
Hey everyone! I’ve been wondering if anyone would be interested in reviving the journal club. There have been quite a few interesting papers on hydrogen-oxidising bacteria lately, and I’d love to discuss them with other… --- ## [Solenoid not responding](https://forum.amybo.org/t/solenoid-not-responding/159)
**Author:** [@Teo](https://forum.amybo.org/u/Teo)\ **Replies:** 4\ **Last updated:** [10 February 2026 15:10 UTC](https://forum.amybo.org/t/solenoid-not-responding/159 "2026-02-10T15:10:01Z")
We at Imperial just got the pioreactor-relay-plugin installed on all our reactors and have one solenoid physically installed on a reactor to test the setup before assembling all of them. Unfortunately, when trying to tes… --- ## [Anode discolouration](https://forum.amybo.org/t/anode-discolouration/155)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 3\ **Last updated:** [5 February 2026 16:09 UTC](https://forum.amybo.org/t/anode-discolouration/155 "2026-02-05T16:09:08Z")
Here’s a rather pretty image of the discolouration from the anode that @Bingqiao returned to me: At the bottom, above the shadow, we see the brown discolouration that we found on the cathode-facing side of the anode.… --- ## [AEP 0.2 Wishlist](https://forum.amybo.org/t/aep-0-2-wishlist/157)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 4\ **Last updated:** [4 February 2026 16:05 UTC](https://forum.amybo.org/t/aep-0-2-wishlist/157 "2026-02-04T16:05:33Z")
A list of potential improvements over Aseptic electroPioreactor v0.0.1 --- ## [How to calibrate optical density](https://forum.amybo.org/t/how-to-calibrate-optical-density/156)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 1\ **Last updated:** [25 January 2026 14:58 UTC](https://forum.amybo.org/t/how-to-calibrate-optical-density/156 "2026-01-25T14:58:21Z")
Hi! Could anyone tell me how to calibrate optical density please? I did not find that in pioreactor docs. --- ## [Two Electrode Systems and Voltage Measurements](https://forum.amybo.org/t/two-electrode-systems-and-voltage-measurements/154)
**Author:** [@Teo](https://forum.amybo.org/u/Teo)\ **Replies:** 3\ **Last updated:** [15 January 2026 13:35 UTC](https://forum.amybo.org/t/two-electrode-systems-and-voltage-measurements/154 "2026-01-15T13:35:40Z")
(Copied over from email correspondence.) I believe I remember a conversation in this group about two versus three electrode systems before winter break. It was in the back of my mind for a while so I decided to do some … --- ## [HOB Culture Medium Ingredients](https://forum.amybo.org/t/hob-culture-medium-ingredients/53)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 14\ **Last updated:** [12 December 2025 13:34 UTC](https://forum.amybo.org/t/hob-culture-medium-ingredients/53 "2025-12-12T13:34:43Z")
Hi @“NarcisP”#9 I've been trying to source the ingredients from your \[2023 paper\](https://doi.org/10.1016/j.jece.2023.111550) so we can start an HOB culture. I'd like the culture medium to be entirely edible by human… --- ## [Waste and media calibration procedures](https://forum.amybo.org/t/waste-and-media-calibration-procedures/152)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 4\ **Last updated:** [11 December 2025 17:47 UTC](https://forum.amybo.org/t/waste-and-media-calibration-procedures/152 "2025-12-11T17:47:55Z")
When I do waste and media calibration, I noticed that the figure is like this. I wonder whether we should subtract the previous mass of water from the current measurement. I remembered that I do not need to do that wh… --- ## [When should I calibrate](https://forum.amybo.org/t/when-should-i-calibrate/151)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 2\ **Last updated:** [8 December 2025 16:34 UTC](https://forum.amybo.org/t/when-should-i-calibrate/151 "2025-12-08T16:34:56Z")
Hi! I wonder when I should calibrate the rotator and stir? For the stir bar, I think I should calibrate it every time before the experiment, since I took the vial for the autoclave. For waste and media rotators, I guess … --- ## [HOB bioreactor assemble](https://forum.amybo.org/t/hob-bioreactor-assemble/147)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 7\ **Last updated:** [4 December 2025 14:09 UTC](https://forum.amybo.org/t/hob-bioreactor-assemble/147 "2025-12-04T14:09:42Z")
Hi! I just tried to assemble the HOB bioreactor and draw the figure to illustrate what I did. I wonder whether I do the wrong thing to all inlet and outlet luer lock. Let’s break the whole system into details. The media … --- ## [HOB autotrophic bioreactor product bottle setting](https://forum.amybo.org/t/hob-autotrophic-bioreactor-product-bottle-setting/146)
**Author:** [@Bingqiao](https://forum.amybo.org/u/Bingqiao)\ **Replies:** 7\ **Last updated:** [3 December 2025 16:16 UTC](https://forum.amybo.org/t/hob-autotrophic-bioreactor-product-bottle-setting/146 "2025-12-03T16:16:01Z")
Hi! I tried to assemble HOB autotrophic bioreactor today. I wonder whether the direction of filter disturb the aseptic level. We need to maintain the inner pressure through a tube connecting to the external environment, … --- ## [16S Community Analysis - DIY or Outsource?](https://forum.amybo.org/t/16s-community-analysis-diy-or-outsource/106)
**Author:** [@gerrit](https://forum.amybo.org/u/gerrit)\ **Replies:** 33\ **Last updated:** [24 November 2025 23:58 UTC](https://forum.amybo.org/t/16s-community-analysis-diy-or-outsource/106 "2025-11-24T23:58:02Z")
Trying to find the most cost effective way to determine what’s growing in our electroPioreactors. Please correct me if I’m wrong, but 16S community analysis appears to be the way to go. So far the best price I have fou… --- ## [Website refresh](https://forum.amybo.org/t/website-refresh/132)
**Author:** [@Amy](https://forum.amybo.org/u/Amy)\ **Replies:** 15\ **Last updated:** [16 October 2025 23:01 UTC](https://forum.amybo.org/t/website-refresh/132 "2025-10-16T23:01:42Z")
Hi all :wave: I’m Amy, I’ve volunteered to help out on the AMY.BO website refresh. I’m a data scientist with a small bit of experience in UX and a very small bit of web dev experience. I’ve started this thread to use fo… --- ## [Welcome to the new forum!](https://forum.amybo.org/t/welcome-to-the-new-forum/62)
**Author:** [@gerrit](https://forum.amybo.org/u/gerrit)\ **Replies:** 1\ **Last updated:** [12 September 2025 12:04 UTC](https://forum.amybo.org/t/welcome-to-the-new-forum/62 "2025-09-12T12:04:21Z")
Hi everyone! You may have noticed that we changed our forum software two weeks ago. We made the switch from Flarum to Discourse, which you’re hopefully more familiar with and should make it easier to contribute. Please … --- ## [Evaluating platinized electrodes for electrolysis](https://forum.amybo.org/t/evaluating-platinized-electrodes-for-electrolysis/71)
**Author:** [@gerrit](https://forum.amybo.org/u/gerrit)\ **Replies:** 11\ **Last updated:** [11 August 2025 11:27 UTC](https://forum.amybo.org/t/evaluating-platinized-electrodes-for-electrolysis/71 "2025-08-11T11:27:50Z")
I decided to run an experiment to test two different platinized electrodes for the in-situ electrolysis, and thought I’d write up the results. I bought a platinized titanium mesh electrode on Ebay for £8.72 and like @Ma… --- ## [Experimental Design Optimisation](https://forum.amybo.org/t/experimental-design-optimisation/140)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 4\ **Last updated:** [25 July 2025 17:00 UTC](https://forum.amybo.org/t/experimental-design-optimisation/140 "2025-07-25T17:00:59Z")
I’m stuck in a rabbit hole literature review and am coming to the conclusion that nobody yet has the optimum trace element solution, let alone experimental electrode or bioreactor configuration. There are so many parame… --- ## [PhD Opportunity](https://forum.amybo.org/t/phd-opportunity/133)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 2\ **Last updated:** [7 July 2025 09:33 UTC](https://forum.amybo.org/t/phd-opportunity/133 "2025-07-07T09:33:01Z")
I received this brilliant PhD opportunity from Sonja Billerbeck at Imperial College’s Bezos Centre for Sustainable Protein this morning: I have an open PhD position (UK home fee) in my lab on precision fermentation with… --- ## [AMY.BO/~You & short URL's](https://forum.amybo.org/t/amy-bo-you-short-urls/137)
**Author:** [@Martin](https://forum.amybo.org/u/Martin)\ **Replies:** 0\ **Last updated:** [30 May 2025 11:08 UTC](https://forum.amybo.org/t/amy-bo-you-short-urls/137 "2025-05-30T11:08:12Z")
Following on from the website refresh discussion I’ve set up a couple of member redirects: AMY.BO/~Martin goes to my link-in-bio AMY.BO/~Gerrit goes to Gerrit’s blog We also have: AMY.BO for our front page AMY.BO/eP … --- ## [Inquiry on Careers and Opportunities in Sustainable Food Production](https://forum.amybo.org/t/inquiry-on-careers-and-opportunities-in-sustainable-food-production/81)
**Author:** [@HannaLewicka](https://forum.amybo.org/u/HannaLewicka)\ **Replies:** 12\ **Last updated:** [15 May 2025 16:02 UTC](https://forum.amybo.org/t/inquiry-on-careers-and-opportunities-in-sustainable-food-production/81 "2025-05-15T16:02:19Z")
Good Afternoon, I hope this message finds you well. My name is Hanna Lewicka, and I am pursuing an MSc in Applied Biosciences and Biotechnology at Imperial College London. I have a strong passion for sustainable food pr… --- ## [Keeping up with HMC Clinic project](https://forum.amybo.org/t/keeping-up-with-hmc-clinic-project/126)
**Author:** [@arlombardo](https://forum.amybo.org/u/arlombardo)\ **Replies:** 42\ **Last updated:** [10 May 2025 04:20 UTC](https://forum.amybo.org/t/keeping-up-with-hmc-clinic-project/126 "2025-05-10T04:20:30Z")
Harvey Mudd College has a clinic team with AMYBO! This is where we will be posting our summarized meeting minutes so the larger AMYBO community can see the progress of our project. Please feel free to reply with comment… [Next page](https://forum.amybo.org/latest.md?page=1)